MFE
primer
3.1
About 3.0
(current)
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Specificity
* Primer sequences (<= 50)
(example)
Number of primer sequences should <= 50, degenerate primers should <= 2.
* Background Database
hg19bs.fa
hg38bs.fasta
Allow mismatch at the 3' end
No
Yes
Accurate but slow, new in version 3.0.
T
m
Min
°C
Minimum T
m
value required for the binding stability between primer and its binding sites.
Size (bp)
From
To
Product size: 0 (min) - 100000 (max).
Concentration of monovalent cations (usually KCl)
mM
Concentration of divalent cations (usually MgCl
2
)
mM
Concentration of dNTPs
mM
Annealing oligo concentration
nM
MFEprimer-3.1
Featured
Specificity analysis
Dimer analysis
Hairpin analysis
Special Thanks / 特别感谢
Bugs? contact Wubin Qu <quwubin@gmail.com>